Enzyme kinetics

Work has been undertaken with Fordyce Davidson (Uni. of Dundee) to investigate the quasi-steady state assumptions in enzyme-reaction kinetics under non homogeneous conditions.

Previous work has in the main concentrated on isolated, spatially uniform reactions. We have investigated the effects of substrate input and diffusion on this formulations, in particular, on the nature and validity of the quasi-stready state assumption (QSSA) for diffusion-driven fronts.
We have shown that provided the Michaelis-Menten constant is sufficiently large then an appropriate QSSA is valid at all points in space and for all times other than in a region which closely tracks the front itself. Moreover, this region shrinks with time.

The validity regions of the QSSA change over time and depend upon nonlocal substrate input

For more information, please contact Graeme Boswell.